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LIS1 antibody

Anti-LIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16993
Anti-LIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16993
Anti-LIS1 antibody used in Western Blot (WB). GTX16993

Cat. No. GTX16993

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all LIS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1.The immunogen is located within amino acids 340 - 390 of LIS1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet activating factor acetylhydrolase 1b regulatory subunit 1 , LIS1 , LIS2 , MDCR , MDS , NudF , PAFAH

Cellular Localization

Cytoplasm, cytoskeleton

Background

This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-LIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16993

GTX16993 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX16993 LIS1 antibody.
Working concentration : 2.5 μg/ml

Anti-LIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16993

GTX16993 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX16993 LIS1 antibody.
Working concentration : 20 μg/ml

Anti-LIS1 antibody used in Western Blot (WB). GTX16993

GTX16993 WB Image

WB analysis of HeLa cell lysate using GTX16993 LIS1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399