GeneTex
United States (US)

LIS1 antibody

GTX16993_34_ICC-IF_20180221_18121410_108.jpg
GTX16993_2314_WB_20180221_18121410_703.jpg
GTX16993_35_ICC-IF_20180221_18121410_566.jpg

Cat No. GTX16993

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , HeLa Cell Lysate
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1.The immunogen is located within amino acids 340 - 390 of LIS1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Platelet Activating Factor Acetylhydrolase 1B Regulatory Subunit 1,Lis1,Lis2,Mdcr,Mds,Nudf,Pafah,Pafah1B1

Background

This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES
GTX16993_34_ICC-IF_20180221_18121410_108.jpg

GTX16993 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX16993 LIS1 antibody.
Working concentration : 2.5 μg/ml

GTX16993_2314_WB_20180221_18121410_703.jpg

GTX16993 WB Image

WB analysis of HeLa cell lysate using GTX16993 LIS1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

GTX16993_35_ICC-IF_20180221_18121410_566.jpg

GTX16993 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX16993 LIS1 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329