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LKB1 antibody

Anti-LKB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130697
Anti-LKB1 antibody used in Western Blot (WB). GTX130697
Anti-LKB1 antibody used in Western Blot (WB). GTX130697
Anti-LKB1 antibody used in Western Blot (WB). GTX130697

Cat. No. GTX130697

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LKB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , A431 , HepG2 , 293T , HeLa

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LKB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 11 , LKB1 , PJS , hLKB1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LKB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130697

GTX130697 IHC-P Image

LKB1 antibody detects LKB1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
LKB1 antibody (GTX130697) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LKB1 antibody used in Western Blot (WB). GTX130697

GTX130697 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LKB1 antibody (GTX130697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LKB1 antibody used in Western Blot (WB). GTX130697

GTX130697 WB Image

LKB1 antibody detects LKB1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LKB1 antibody (GTX130697) diluted at a dilution of 1:1000.

Anti-LKB1 antibody used in Western Blot (WB). GTX130697

GTX130697 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 30717766

REFERENCE

Application Reference

Jiang WP et al. Antioxidants (Basel) 2021; 10 (6) Sanghuangporus sanghuang Mycelium Prevents Paracetamol-Induced Hepatotoxicity through Regulating the MAPK/NF-κB, Keap1/Nrf2/HO-1, TLR4/PI3K/Akt, and CaMKKβ/LKB1/AMPK Pathways and Suppressing Oxidative Stress and Inflammation.
Application : WB
Reactivity : Mouse
Tuo L et al. J Exp Clin Cancer Res 2019; 38 (1) : 50 PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169