GeneTex
United States (US)

LKB1 (phospho Ser428) antibody

LKB1-phospho-Ser428-antibody-GTX30765-WB-1_18121411_404.jpg
LKB1-phospho-Ser428-antibody_IHC_GTX30765-1_18121411_656.jpg

Cat No. GTX30765

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For ELISA: Use at a dilution of 1:500-1:1,000. For IHC-P: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

49 kDa. ( Note )

Positive Control

Human breast carcinoma tissue, CV-1 cells treated with forsklin

Specificity/Sensitivity

Detects endogenous levels of LKB1 only when phosphorylated at serine 428
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide - Human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C)

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine/Threonine Kinase 11,Lkb1,Pjs,Hlkb1,Stk11

Background

LKB1, a member of the serine/threonine kinase family, is a tumor suppressor. Physically associates with p53, it regulates specific p53 dependent apoptosis pathways. LKB1 protein is present in both the cytoplasm and nucleus of living cells and translocates to mitochondria during apoptosis. Restoring LKB1 activity into cancer cell lines defective for its expression results in a G1 cell cycle arrest.Mutations in LKB1 gene are associated with Peutz Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. LKB1 functions as a master upstream protein kinase, regulating AMPK related kinases as well as AMPK. In vitro, LKB1 has been observed to directly phosphorylate threonine 172 of AMPKa, an event that is correlated with activation of this kinase. LKB1 has also been found to play a role in chromatin remodeling, cell cycle arrest, Wnt signaling, cell polarity, and energy metabolism.

Database

Research Area

DATA IMAGES
LKB1-phospho-Ser428-antibody-GTX30765-WB-1_18121411_404.jpg

GTX30765 WB Image

WB analysis of extracts from CV-1 cells treated with forsklin. Lane 1.) Using antibody and no peptide. Lane 2.) Using antibody preincubated with peptide.

LKB1-phospho-Ser428-antibody_IHC_GTX30765-1_18121411_656.jpg

GTX30765 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LKB1 (phospho-Ser428) antibody