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LMAN1 antibody [N1C1]

Anti-LMAN1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114530
Anti-LMAN1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114530
Anti-LMAN1 antibody [N1C1] used in Western Blot (WB). GTX114530

Cat. No. GTX114530

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LMAN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LMAN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10634311

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lectin, mannose binding 1 , ERGIC-53 , ERGIC53 , F5F8D , FMFD1 , MCFD1 , MR60 , gp58

Cellular Localization

Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein , Golgi apparatus membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LMAN1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114530

GTX114530 IHC-P Image

LMAN1 antibody [N1C1] detects LMAN1 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
LMAN1 antibody [N1C1] (GTX114530) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LMAN1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114530

GTX114530 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using LMAN1(GTX114530) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LMAN1 antibody [N1C1] used in Western Blot (WB). GTX114530

GTX114530 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114530 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169