APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lectin, mannose binding 1 , ERGIC-53 , ERGIC53 , F5F8D , FMFD1 , MCFD1 , MR60 , gp58
Background
The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015]
Database
Research Area
DATA IMAGES
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GTX64719 WB Image
WB analysis of various sample lysates using GTX64719 LMAN1 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX64719 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX64719 LMAN1 antibody. Blue : DAPI Dilution : 1:100
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GTX64719 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX64719 LMAN1 antibody. Blue : DAPI Dilution : 1:100
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GTX64719 ICC/IF Image
ICC/IF analysis of C6 cells using GTX64719 LMAN1 antibody. Blue : DAPI Dilution : 1:100
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REFERENCE
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REVIEW
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