Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LMO4 antibody (GTX129616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

LMO4 antibody detects LMO4 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human LMO4-transfected 293T cells
12 % SDS-PAGE
LMO4 antibody (GTX129616) dilution: 1:5000

LMO4 antibody detects LMO4 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LMO4 antibody (GTX129616) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min