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LMX1B antibody

Anti-LMX1B antibody used in IHC (Frozen sections) (IHC-Fr). GTX129831
Anti-LMX1B antibody used in Western Blot (WB). GTX129831

Cat No. GTX129831

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse skeletal muscle

Predict Reactivity

Human, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human LMX1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LIM homeobox transcription factor 1 beta , LMX1.2 , NPS1

Cellular Localization

Nucleus

Background

This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-LMX1B antibody used in IHC (Frozen sections) (IHC-Fr). GTX129831

GTX129831 IHC-Fr Image

LMX1B antibody detects LMX1B protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: LMX1B protein stained by LMX1B antibody (GTX129831) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LMX1B antibody used in Western Blot (WB). GTX129831

GTX129831 WB Image

LMX1B antibody detects LMX1B protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with LMX1B antibody (GTX129831) at a dilution of 1:2000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169