APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , A20 Cell Lysate(GTX27180)
Product Note
At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B.The immunogen is located within amino acids 280 - 330 of LMX1B.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
LIM homeobox transcription factor 1 beta , LMX1.2 , NPS1
Cellular Localization
Nucleus
Background
This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Database
Research Area
DATA IMAGES
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GTX31508 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX31508 LMX1B antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin
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GTX31508 WB Image
WB analysis of A-20 cell lysate using GTX31508 LMX1B antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31508 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX31508 LMX1B antibody.
Working concentration : 5 μg/ml
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GTX31508 IHC-P Image
IHC-P analysis of human brain tissue using GTX31508 LMX1B antibody.
Working concentration : 5 μg/ml
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GTX31508 IHC-P Image
IHC-P analysis of human brain tissue using GTX31508 LMX1B antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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