APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 20 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lung Tissue Lysate(GTX27917) , Human Lung Tissue Slide
Product Note
At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP.The immunogen is located within amino acids 900 - 950 of LNPEP.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
leucyl and cystinyl aminopeptidase , CAP , IRAP , P-LAP , PLAP
Cellular Localization
Cell membrane
Background
This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX32096 IHC-P Image
IHC-P analysis of human lung tissue using GTX32096 LNPEP antibody.
Working concentration : 20 μg/ml
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GTX32096 WB Image
WB analysis of human lung tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX32096 LNPEP antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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