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LOX antibody [5C4]

Overexpression
Anti-LOX antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84188
Anti-LOX antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84188
Anti-LOX antibody [5C4] used in Western Blot (WB). GTX84188
Anti-LOX antibody [5C4] used in Western Blot (WB). GTX84188

Cat. No. GTX84188

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Dog
Package
100 μl ($519)
See all LOX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysyl oxidase , AAT10

Cellular Localization

Secreted

Background

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.

Database

Research Area

DATA IMAGES

Anti-LOX antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84188

GTX84188 IHC-P Image

IHC-P analysis of human colon tissue using GTX84188 LOX antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-LOX antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84188

GTX84188 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with LOX plasmid using GTX84188 LOX antibody [5C4].

Anti-LOX antibody [5C4] used in Western Blot (WB). GTX84188

GTX84188 WB Image

WB analysis of various cell lines using GTX84188 LOX antibody [5C4].
Loading : 35 ug per lane

Anti-LOX antibody [5C4] used in Western Blot (WB). GTX84188

GTX84188 WB Image

Overexpression

WB analysis of HEK293T cells transfected with LOX plasmid (Right) or empty vector (Left) for 48 hrs using GTX84188 LOX antibody [5C4].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519