GeneTex
United States (US)

LOXL2 antibody

KO/KD Overexpression
GTX105085_42424_20160420_IHC-P_M_19011506_667.jpg
GTX105085_42123_20170119_WB_KO_watermark_19011506_465.jpg
GTX105085_42389_20160511_IFA_19011506_496.jpg
GTX105085_41528_IHC_19011506_478.jpg
GTX105085_42018_20150814_IHC_19011506_714.jpg
GTX105085_43187_20180420_WB_19021719_789.jpg
GTX105085_43236_20190116_ICC_IF_19030801_435.jpg
GTX105085_42221_20151203_WB_B_19011506_173.jpg
GTX105085_42046_20160825_WB_19011506_675.jpg
GTX105085_42123_20161110_WB_M_lung_19011506_323.jpg

Cat No. GTX105085

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

HeLa , MDA-MB-231 , U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2 , mouse lung

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lysyl Oxidase Like 2,Lor,Lor2,Ws9-14,Loxl2

Cellular Localization

Secreted , extracellular space

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105085_42424_20160420_IHC-P_M_19011506_667.jpg

GTX105085 IHC-P Image

LOXL2 antibody detects secreted LOXL2 protein in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
LOXL2 antibody (GTX105085) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105085_42123_20170119_WB_KO_watermark_19011506_465.jpg

GTX105085 WB Image

KO/KD

Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX105085_42389_20160511_IFA_19011506_496.jpg

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX105085_41528_IHC_19011506_478.jpg

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
LOXL2 antibody (GTX105085) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105085_42018_20150814_IHC_19011506_714.jpg

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
LOXL2 antibody (GTX105085) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105085_43187_20180420_WB_19021719_789.jpg

GTX105085 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105085_43236_20190116_ICC_IF_19030801_435.jpg

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX105085_42221_20151203_WB_B_19011506_173.jpg

GTX105085 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

GTX105085_42046_20160825_WB_19011506_675.jpg

GTX105085 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105085_42123_20161110_WB_M_lung_19011506_323.jpg

GTX105085 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf