LOXL2 antibody

Name: LOXL2 antibody
Brand: GeneTex
SKU: GTX105085
Price: 399.00 USD

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085

Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085

Anti-LOXL2 antibody used in ELISA (ELISA). GTX105085

Cat. No. GTX105085

Reference 18

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all LOXL2 products

View product citations for antibody GTX105085 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
ELISA	Assay dependent

Not tested in other applications.

Observed MW

100 (proform), 65 (cleaved form) kDa.

Positive Control

U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2

Product Note

Mouse reactivity has been validated by IHC-P only, not yet been tested by other applications.

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950827

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysyl oxidase like 2 , LOR , LOR2 , WS9-14

Cellular Localization

Secreted , extracellular space

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Database

Gene ID: 4017 LOXL2
UniProt: Q9Y4K0 LOXL2

Research Area

DATA IMAGES

GTX105085 WB Image

Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:3000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105085 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (GTX136930-pro) (44.94-0.7 nM). Coated protein was probed with LOXL2 antibody (GTX105085) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105085 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

GTX105085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

GTX105085 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105085 WB Image

Human LOXL2 protein, His tag (1 μg, GTX136930-pro) was separated by 10% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.