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LOXL2 antibody

Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085
Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085
Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

Cat No. GTX105085

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

HeLa , MDA-MB-231 , U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2 , mouse lung

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lysyl oxidase like 2 , LOR , LOR2 , WS9-14

Cellular Localization

Secreted , extracellular space

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 21732535

Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169