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LOXL2 antibody

Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085
Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085
Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in Western Blot (WB). GTX105085
Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085

Cat No. GTX105085

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

HeLa , MDA-MB-231 , U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2 , mouse lung

Product Note

Mouse reactivity has been validated by IHC-P only, not yet been tested by other applications.

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950827

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysyl oxidase like 2 , LOR , LOR2 , WS9-14

Cellular Localization

Secreted , extracellular space

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:3000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LOXL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105085

GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LOXL2 antibody used in Western Blot (WB). GTX105085

GTX105085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 21732535

Anti-LOXL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105085

GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

REVIEW

LOXL2 antibody Cat No. GTX105085

Rating ( Average 5 based on 9 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 5 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28273952
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28273952
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2017.PMID: 28449718
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2017.PMID: 28449718
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2017.PMID: 28449718
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2019.PMID: 31086173
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2019.PMID: 31086173
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Oncol in 2020.PMID: 32211324
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Oncol in 2020.PMID: 32211324
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf