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LPL antibody

Anti-LPL antibody used in Western Blot (WB). GTX101125
Anti-LPL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101125
Anti-LPL antibody used in IHC (Paraffin sections) (IHC-P). GTX101125
Anti-LPL antibody used in Western Blot (WB). GTX101125

Cat. No. GTX101125

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Sheep, Chicken
Package
100 μl ($399),
25 μl ($169)
See all LPL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LPL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950830

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lipoprotein lipase , HDLCQ11 , LIPD

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor , Secreted

Background

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LPL antibody used in Western Blot (WB). GTX101125

GTX101125 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody (GTX101125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LPL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101125

GTX101125 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using LPL(GTX101125) antibody at 1:200 dilution.

Anti-LPL antibody used in IHC (Paraffin sections) (IHC-P). GTX101125

GTX101125 IHC-P Image

LPL antibody detects secreted LPL protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
LPL stained by LPL antibody (GTX101125) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LPL antibody used in Western Blot (WB). GTX101125

GTX101125 WB Image

NIH-3T3 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody (GTX101125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Shimizu K et al. PLoS One 2022; 17 (9) : e0274297 Liver-specific overexpression of lipoprotein lipase improves glucose metabolism in high-fat diet-fed mice.
Application : WB, IHC-P
Reactivity : Mouse
Sandoval C et al. Domest Anim Endocrinol 2021; 74 : 106556 Effect of maternal nutrient restriction on expression of glucose transporters (SLC2A4 and SLC2A1) and insulin signaling in skeletal muscle of SGA and Non-SGA sheep fetuses.
Application : WB
Reactivity : Sheep
Little HC et al. FASEB J 2019; : fj201900520R Myonectin deletion promotes adipose fat storage and reduces liver steatosis.
Application : WB
Reactivity : Mouse
Qiao L et al. Diabetes 2015; 64 (9) : 3111-20 Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice.
Application : WB
Reactivity : Mouse
Kosla J et al. Gene 2013; 513 (1) : 90-100 Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts.
Application : WB
Reactivity : Chicken
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169