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LPL antibody

Anti-LPL antibody used in Western Blot (WB). GTX101125
Anti-LPL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101125
Anti-LPL antibody used in Western Blot (WB). GTX101125
Anti-LPL antibody used in IHC (Paraffin sections) (IHC-P). GTX101125

Cat. No. GTX101125

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Sheep, Chicken
Package
100 μl ($399),
25 μl ($169)
See all LPL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LPL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950830

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lipoprotein lipase , HDLCQ11 , LIPD

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor , Secreted

Background

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LPL antibody used in Western Blot (WB). GTX101125

GTX101125 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody (GTX101125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LPL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101125

GTX101125 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using LPL(GTX101125) antibody at 1:200 dilution.

Anti-LPL antibody used in Western Blot (WB). GTX101125

GTX101125 WB Image

NIH-3T3 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LPL antibody (GTX101125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LPL antibody used in IHC (Paraffin sections) (IHC-P). GTX101125

GTX101125 IHC-P Image

LPL antibody detects secreted LPL protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
LPL stained by LPL antibody (GTX101125) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Sandoval C et al. Domest Anim Endocrinol 2021; 74 : 106556 Effect of maternal nutrient restriction on expression of glucose transporters (SLC2A4 and SLC2A1) and insulin signaling in skeletal muscle of SGA and Non-SGA sheep fetuses.
Application : WB
Reactivity : Sheep
Little HC et al. FASEB J 2019; : fj201900520R Myonectin deletion promotes adipose fat storage and reduces liver steatosis.
Application : WB
Reactivity : Mouse
Qiao L et al. Diabetes 2015; 64 (9) : 3111-20 Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice.
Application : WB
Reactivity : Mouse
Kosla J et al. Gene 2013; 513 (1) : 90-100 Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts.
Application : WB
Reactivity : Chicken
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169