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LRIG1 antibody [C2C3], C-term

KO/KD

Cat. No. GTX119485

Reference 3
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LRIG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by customer review data.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human LRIG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164800

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich repeats and immunoglobulin like domains 1 , LIG-1 , LIG1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Act as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.

Database

Research Area

REFERENCE

Application Reference

Han-Lin Hsu et al. Int J Mol Sci 2021; 22 (18) Aryl Hydrocarbon Receptor Defect Attenuates Mitogen-Activated Signaling through Leucine-Rich Repeats and Immunoglobulin-like Domains 1 (LRIG1)-Dependent EGFR Degradation.
Application : IP
Reactivity : Mouse
Yokdang N et al. Oncogene 2016; 35 (22) : 2932-47 LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells.
N Yokdang et al. Oncogene 2015; LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells[Epub ahead]
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

LRIG1 antibody [C2C3], C-term Cat. No. GTX119485

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-LRIG1 antibody [C2C3], C-term used in Western Blot (WB). GTX119485
Date : Anonymous submitted on 06-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-453 cells
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane1: MDA-MB-453 cells, Lane2: knockdown of human LRIG1 in MDA-MB-453 cells
Figure Note : Star Review and image courtesy of Nucharee Yokdang (University of California, Davis)
Anti-LRIG1 antibody [C2C3], C-term used in Western Blot (WB). GTX119485
Date : Anonymous submitted on 06-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Star Review and image courtesy of Nucharee Yokdang (University of California, Davis)
Anti-LRIG1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX119485
Date : Anonymous submitted on 15-Sep-2015
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : MDA-MB-231 LRIG1 overexpressing Xenograft tissue
Blocking : 5% Normal Goat Serum in PBST, 25°C, 0.67Hr
Fixation : 4% paraformaldehyde, 4°C, 24Hr
Permeabilization /Antigen retrieval : Triton-X solution/ Sodium-Citrate Buffer, 30minHr
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : Star Review and image courtesy of Nucharee Yokdang (University of California, Davis)
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169