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LRP130 antibody [HL2056]

Recombinant antibody KO/KD Orthogonal
Anti-LRP130 antibody [HL2056] used in Western Blot (WB). GTX637960
Anti-LRP130 antibody [HL2056] used in Western Blot (WB). GTX637960
Anti-LRP130 antibody [HL2056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637960

Cat. No. GTX637960

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2056

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LRP130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human LRP130

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich pentatricopeptide repeat containing , CLONE-23970 , GP130 , LRP130 , LSFC

Cellular Localization

Mitochondrion,nucleoplasm,Nucleus inner membrane,Nucleus outer membrane

Background

This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-LRP130 antibody [HL2056] used in Western Blot (WB). GTX637960

GTX637960 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [HL2056] (GTX637960) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRP130 antibody [HL2056] used in Western Blot (WB). GTX637960

GTX637960 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [HL2056] (GTX637960) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-LRP130 antibody [HL2056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637960

GTX637960 ICC/IF Image

LRP130 antibody [HL2056] detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LRP130 stained by LRP130 antibody [HL2056] (GTX637960) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations