Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N2N3] (GTX115351) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
5% SDS PAGE
GTX115351 diluted at 1:1000

LRP130 antibody [N2N3] detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: LRP130 protein stained by LRP130 antibody [N2N3] (GTX115351) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using LRP130(GTX115351) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min