LRP130 antibody [N3C2], Internal

Name: LRP130 antibody [N3C2], Internal
Brand: GeneTex
SKU: GTX109558
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558

Anti-LRP130 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109558

Anti-LRP130 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109558

Cat. No. GTX109558

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all LRP130 products

View product citations for antibody GTX109558 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950836

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich pentatricopeptide repeat containing , CLONE-23970 , GP130 , LRP130 , LSFC

Cellular Localization

Mitochondrion , Nucleus , nucleoplasm , Nucleus inner membrane , Nucleus outer membrane

Background

This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. [provided by RefSeq]

Database

Gene ID: 10128 LRPPRC
UniProt: P42704 LRPPRC

Research Area

DATA IMAGES

GTX109558 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109558 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109558 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 (GTX109558) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109558 ICC/IF Image

LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX109558 IHC-P Image

LRP130 antibody [N3C2], Internal detects LRP130 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109558 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HeLa
5% SDS PAGE
GTX109558 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yu Y et al. Clin Transl Med 2024; 14 (2) : e1583 LRPPRC promotes glycolysis by stabilising LDHA mRNA and its knockdown plus glutamine inhibitor induces synthetic lethality via m(6) A modification in triple-negative breast cancer.

Nishio T et al. Clin Chim Acta 2017; (Epub) Immunohistochemical expression analysis of leucine-rich PPR-motif-containing protein (LRPPRC), a candidate colorectal cancer biomarker identified by shotgun proteomics using iTRAQ.

Application : IHC-P

Reactivity : Human

Gaweda-Walerych K et al. Neurobiol Aging 2016; 47 : 127-138 Parkinson's disease-related gene variants influence pre-mRNA splicing processes.

Application : WB

Reactivity : Human

Kasashima K et al. Exp Cell Res 2011; 317 (2) : 210-20 Human mitochondrial transcription factor A is required for the segregation of mitochondrial DNA in cultured cells.

Application : ICC/IF

Reactivity : Human

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