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LRP130 antibody [N3C2], Internal

Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558
Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558
Anti-LRP130 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109558
Anti-LRP130 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109558
Anti-LRP130 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109558
Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558

Cat. No. GTX109558

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950836

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich pentatricopeptide repeat containing , CLONE-23970 , GP130 , LRP130 , LSFC

Cellular Localization

Mitochondrion , Nucleus , nucleoplasm , Nucleus inner membrane , Nucleus outer membrane

Background

This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558

GTX109558 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558

GTX109558 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRP130 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109558

GTX109558 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 (GTX109558) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LRP130 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109558

GTX109558 ICC/IF Image

LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-LRP130 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109558

GTX109558 IHC-P Image

LRP130 antibody [N3C2], Internal detects LRP130 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LRP130 antibody [N3C2], Internal used in Western Blot (WB). GTX109558

GTX109558 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HeLa
5% SDS PAGE
GTX109558 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

LRP130 antibody [N3C2], Internal Cat. No. GTX109558

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : mouse kidney cells
Blocking : 10% horse serum, 25°C, 0.5Hr
Fixation : 2% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, 25°C, 0.5hrHr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169