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LRP4 antibody [HL2080]

Recombinant antibody
Anti-LRP4 antibody [HL2080] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637988
Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988
Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988
Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988
Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988

Cat. No. GTX637988

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2080

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all LRP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

212 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant fragment of mouse LRP4

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

low density lipoprotein receptor-related protein 4 , 6430526J12Rik , D230026E03 , Megf7 , mdig

Cellular Localization

Cell membrane

Background

Enables protein homodimerization activity and receptor tyrosine kinase binding activity. Involved in several processes, including positive regulation of presynaptic membrane organization; positive regulation of skeletal muscle acetylcholine-gated channel clustering; and synapse organization. Acts upstream of or within several processes, including animal organ development; embryonic digit morphogenesis; and regionalization. Located in neuromuscular junction and plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; eye; and genitourinary system. Used to study sclerosteosis 2. Human ortholog(s) of this gene implicated in Cenani-Lenz syndactyly syndrome; congenital myasthenic syndrome 17; and sclerosteosis 2. Orthologous to human LRP4 (LDL receptor related protein 4). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-LRP4 antibody [HL2080] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637988

GTX637988 ICC/IF Image

LRP4 antibody [HL2080] detects LRP4 protein at cell membrane and nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LRP4 stained by LRP4 antibody [HL2080] (GTX637988) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988

GTX637988 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988

GTX637988 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988

GTX637988 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRP4 antibody [HL2080] used in Western Blot (WB). GTX637988

GTX637988 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations