APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 2-4μg/ml |
|
Note :
IHC-P
Human Liver shows weak cytoplasm staining in some hepatocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89521-PEP).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-ERVEKTTGDKRT, from the internal region of the protein sequence according to NP_002326.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
LDL receptor related protein 5 , BMND1 , EVR1 , EVR4 , HBM , LR3 , LRP-5 , LRP-7 , LRP7 , OPPG , OPS , OPTA1 , PCLD4 , VBCH2
Cellular Localization
Membrane , Endoplasmic reticulum
Background
This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
|
GTX89521 IHC-P Image
IHC-P analysis of human liver using GTX89521 LRP5 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
|
|
|
GTX89521 WB Image
WB analysis of HepG2 lysate using GTX89521 LRP5 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for LRP5 antibody, Internal (GTX89521). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LRP5 antibody, Internal (GTX89521). Be the first to share your experience with this product.
