GeneTex

  • Country / Location Selection

United States (US)

LRP6 (phospho Ser1490) antibody

Orthogonal
Anti-LRP6 (phospho Ser1490) antibody used in Western Blot (WB). GTX130421
Anti-LRP6 (phospho Ser1490) antibody used in Western Blot (WB). GTX130421

Cat. No. GTX130421

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LRP6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

180 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1490 of human LRP6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886260

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LDL receptor related protein 6 , ADCAD2 , STHAG7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The LRP6 gene encodes a member of the low density lipoprotein receptor (LDLR) gene family, which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDLR proteins are comprised of the same basic structural motifs: an extracellular domain that contains LDLR binding repeats and EGF repeats with associated spacer domains containing the YWTD motif; a single transmembrane-spanning domain; and a C-terminal cytoplasmic domain that generally contains at least 1 copy of the NPXY motif (Brown et al., 1998 [PubMed 9704021]). LRP6 and LRP5 (MIM 603506) function as coreceptors for WNT (MIM 164820) ligands and thus play a role in WNT signaling, which is important in a wide variety of biologic processes during pre- and postnatal life in invertebrates and vertebrates (Kokubu et al., 2004 [PubMed 15469977]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LRP6 (phospho Ser1490) antibody used in Western Blot (WB). GTX130421

GTX130421 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP6 phospho S1490 antibody (GTX130421) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)

Anti-LRP6 (phospho Ser1490) antibody used in Western Blot (WB). GTX130421

GTX130421 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP6 (phospho Ser1490) antibody (GTX130421) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for LRP6 (phospho Ser1490) antibody (GTX130421). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for LRP6 (phospho Ser1490) antibody (GTX130421). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169