APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse kidney , rat kidney , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human LRPAP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11165591
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
LDL receptor related protein associated protein 1 , A2MRAP , A2RAP , HBP44 , MRAP , MYP23 , RAP , alpha-2-MRAP
Cellular Localization
Endoplasmic reticulum , Cytoplasm , Cell surface
Background
Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
Database
Research Area
DATA IMAGES
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/templates/default/js/lightbox/images/loading.gif "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
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GTX104033 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] (GTX104033) diluted at 1:1000.
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GTX104033 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] (GTX104033) diluted at 1:1000.
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GTX104033 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] (GTX104033) diluted at 1:1000.
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GTX104033 ICC/IF Image
RAP antibody [N1C3] detects RAP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAP protein stained by RAP antibody [N1C3] (GTX104033) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX104033 IHC-P Image
RAP antibody [N1C3] detects RAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAP antibody [N1C3] (GTX104033) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for LRPAP1 antibody [N1C3] (GTX104033). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LRPAP1 antibody [N1C3] (GTX104033). Be the first to share your experience with this product.
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/upload/website/prouct_img/small/GTX104033/GTX104033_40527_20160428_WB_w_23060120_207.webp "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/upload/website/prouct_img/small/GTX104033/GTX104033_40527_20151119_WB_Merged_w_23060120_602.webp "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/upload/website/prouct_img/small/GTX104033/GTX104033_40527_20151119_WB_w_23060120_942.webp "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104033](/upload/website/prouct_img/small/GTX104033/GTX104033_40527_20160727_IFA_w_23060120_904.webp "Anti-LRPAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104033](/upload/website/prouct_img/small/GTX104033/GTX104033_40527_20160316_IHC-P_R_w_23060120_607.webp "Anti-LRPAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/upload/website/prouct_img/normal/GTX104033/GTX104033_40527_20160428_WB_w_23060120_207.webp "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/upload/website/prouct_img/normal/GTX104033/GTX104033_40527_20151119_WB_Merged_w_23060120_602.webp "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033](/upload/website/prouct_img/normal/GTX104033/GTX104033_40527_20151119_WB_w_23060120_942.webp "Anti-LRPAP1 antibody [N1C3] used in Western Blot (WB). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104033](/upload/website/prouct_img/normal/GTX104033/GTX104033_40527_20160727_IFA_w_23060120_904.webp "Anti-LRPAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104033")
![Anti-LRPAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104033](/upload/website/prouct_img/normal/GTX104033/GTX104033_40527_20160316_IHC-P_R_w_23060120_607.webp "Anti-LRPAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104033")