Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LRRC1 antibody [C1C3] (GTX120541) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using LRRC1(GTX120541) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX120541 diluted at 1:1000