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LRRC59 antibody

Anti-LRRC59 antibody used in Western Blot (WB). GTX122902
Anti-LRRC59 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122902
Anti-LRRC59 antibody used in Western Blot (WB). GTX122902
Anti-LRRC59 antibody used in IHC (Paraffin sections) (IHC-P). GTX122902

Cat No. GTX122902

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRRC59. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177630

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

leucine rich repeat containing 59 , PRO1855 , p34

Cellular Localization

Microsome membrane; Single-pass type II membrane protein , Endoplasmic reticulum membrane; Single-pass type II membrane protein

Database

Research Area

DATA IMAGES

Anti-LRRC59 antibody used in Western Blot (WB). GTX122902

GTX122902 WB Image

Wild-type (WT) and LRRC59 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LRRC59 antibody (GTX122902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRRC59 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122902

GTX122902 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LRRC59(GTX122902) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-LRRC59 antibody used in Western Blot (WB). GTX122902

GTX122902 WB Image

Sample (30 μg of whole cell lysate)
A: A431
B: HeLa
C: HepG2
10% SDS PAGE
GTX122902 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRRC59 antibody used in IHC (Paraffin sections) (IHC-P). GTX122902

GTX122902 IHC-P Image

LRRC59 antibody detects LRRC59 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
LRRC59 antibody (GTX122902) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

LRRC59 antibody Cat No. GTX122902

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20 μg
Blocking : 5 % skim milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169