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LRRC59 antibody

Anti-LRRC59 antibody used in Western Blot (WB). GTX123532
Anti-LRRC59 antibody used in Western Blot (WB). GTX123532
Anti-LRRC59 antibody used in IHC (Paraffin sections) (IHC-P). GTX123532
Anti-LRRC59 antibody used in IHC (Paraffin sections) (IHC-P). GTX123532

Cat No. GTX123532

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRRC59. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885588

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

leucine rich repeat containing 59 , PRO1855 , p34

Cellular Localization

Microsome membrane; Single-pass type II membrane protein , Endoplasmic reticulum membrane; Single-pass type II membrane protein

Database

Research Area

DATA IMAGES

Anti-LRRC59 antibody used in Western Blot (WB). GTX123532

GTX123532 WB Image

Wild-type (WT) and LRRC59 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LRRC59 antibody (GTX123532) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LRRC59 antibody used in Western Blot (WB). GTX123532

GTX123532 WB Image

LRRC59 antibody detects LRRC59 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
LRRC59 antibody (GTX123532) dilution: 1:1000

Anti-LRRC59 antibody used in IHC (Paraffin sections) (IHC-P). GTX123532

GTX123532 IHC-P Image

LRRC59 antibody detects LRRC59 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
LRRC59 antibody (GTX123532) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LRRC59 antibody used in IHC (Paraffin sections) (IHC-P). GTX123532

GTX123532 IHC-P Image

LRRC59 antibody detects LRRC59 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
LRRC59 antibody (GTX123532) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169