GeneTex
United States (US)

LRRK2 antibody [N1], N-term

GTX113065_40114_WB_18121410_374.jpg
GTX113065_40114_IHC_18121410_873.jpg
GTX113065_40114_WB_M_kidney_18121410_608.jpg

Cat No. GTX113065

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

286 kDa. ( Note )

Positive Control

SK-N-SH , mouse kidney

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LRRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Leucine Rich Repeat Kinase 2,Aura17,Dardarin,Park8,Ripk7,Roco2,Lrrk2

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113065_40114_WB_18121410_374.jpg

GTX113065 WB Image

LRRK2 antibody [N1], N-term detects LRRK2 protein by Western blot analysis.
A. 30 µg SK-N-SH whole cell lysate/extract
5 % SDS-PAGE
LRRK2 antibody [N1], N-term (GTX113065) dilution: 1:500

GTX113065_40114_IHC_18121410_873.jpg

GTX113065 IHC-P Image

LRRK2 antibody [N1], N-term detects LRRK2 protein at cytosol on human brain by immunohistochemical analysis.
Sample: Paraffin-embedded human brain.
LRRK2 antibody [N1], N-term (GTX113065) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113065_40114_WB_M_kidney_18121410_608.jpg

GTX113065 WB Image

Sample (50 ug of whole cell lysate)
A: mouse kidney
5% SDS PAGE
GTX113065 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169