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LRRK2 antibody [N3], Internal

Comparison
Anti-LRRK2 antibody [N3], Internal used in Western Blot (WB). GTX113067
Anti-LRRK2 antibody [N3], Internal used in Western Blot (WB). GTX113067
Anti-LRRK2 antibody [N3], Internal used in IHC (Paraffin sections) (IHC-P). GTX113067
Anti-LRRK2 antibody [N3], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113067

Cat. No. GTX113067

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LRRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

286 kDa. ( Note )

Positive Control

IMR32 , mouse cerebellum

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LRRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich repeat kinase 2 , AURA17 , DARDARIN , PARK8 , RIPK7 , ROCO2

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LRRK2 antibody [N3], Internal used in Western Blot (WB). GTX113067

GTX113067 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with LRRK2 antibody [N3], Internal (GTX113067) diluted at 1:500 and competitor's antibody (#13046) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LRRK2 antibody [N3], Internal used in Western Blot (WB). GTX113067

GTX113067 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
5% SDS PAGE
GTX113067 diluted at 1:1000

Anti-LRRK2 antibody [N3], Internal used in IHC (Paraffin sections) (IHC-P). GTX113067

GTX113067 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using LRRK2(GTX113067) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LRRK2 antibody [N3], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113067

GTX113067 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using LRRK2(GTX113067) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169