GeneTex
United States (US)

LRRK2 antibody

GTX30678_1622_IP_18121410_936.jpg
GTX30678_340_IHC-P_18121410_295.jpg
GTX30678_1478_WB_18121410_873.jpg
GTX30678_1479_WB_18121410_738.jpg
GTX30678_239_ICC-IF_18121410_538.jpg
GTX30678_238_ICC-IF_18121410_340.jpg

Cat No. GTX30678

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Bovine, Caenorhabditis elegans
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000 - 1:5000
ICC/IF 1:1000 - 1:2000
IHC-P 1:500 - 1:1000
IHC-Fr Assay dependent
FACS 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

286 kDa. ( Note )

Predict Reactivity

Rat, Drosophila(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AURA17 antibody , DARDARIN antibody , PARK8 antibody , RIPK7 antibody , ROCO2 antibody

Cellular Localization

Cytoplasm,Membrane, Peripheral membrane protein

Background

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq]

Research Area

DATA IMAGES
GTX30678_1622_IP_18121410_936.jpg

GTX30678 IP Image

IP analysis of SH-SY5Y cell lysate using GTX30678 LRRK2 antibody.
Lane 1 : Rabbit IgG
Lane 2 and 3 : GTX30678

GTX30678_340_IHC-P_18121410_295.jpg

GTX30678 IHC-P Image

IHC-P analysis of human brain tissue using GTX30678 LRRK2 antibody.

GTX30678_1478_WB_18121410_873.jpg

GTX30678 WB Image

WB analysis of bovine brain membrane extract using GTX30678 LRRK2 antibody.
Loading : 50μg

GTX30678_1479_WB_18121410_738.jpg

GTX30678 WB Image

WB analysis of HeLa cell lysate (in RIPA buffer) using GTX30678 LRRK2 antibody.
Dilution : 1:5000

GTX30678_239_ICC-IF_18121410_538.jpg

GTX30678 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX30678 LRRK2 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200

GTX30678_238_ICC-IF_18121410_340.jpg

GTX30678 ICC/IF Image

ICC/IF analysis of mouse CAD cells overexpressing human LRRK2 using GTX30678 LRRK2 antibody.
Dilution : 1:2000