APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:2000 - 1:5000 |
| 1:1000 - 1:2000 |
| 1:500 - 1:1000 |
| Assay dependent |
| 1:200 |
| 1:10 - 1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Drosophila(>80% identity)
PROPERTIES
Form
Liquid
Buffer
Tris-Citrate/Phosphate
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
leucine rich repeat kinase 2 , AURA17 , DARDARIN , PARK8 , RIPK7 , ROCO2
Cellular Localization
Cytoplasm,Mitochondrion,Golgi apparatus,Cell projection, dendrite,Endoplasmic reticulum,Endosome,Lysosome,Mitochondrion outer membrane,Mitochondrion inner membrane,Mitochondrion matrix
Background
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX30678 ICC/IF Image
ICC/IF analysis of mouse CAD cells overexpressing human LRRK2 using GTX30678 LRRK2 antibody.
Dilution : 1:2000
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GTX30678 WB Image
WB analysis of HeLa cell lysate (in RIPA buffer) using GTX30678 LRRK2 antibody.
Dilution : 1:5000
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GTX30678 WB Image
WB analysis of bovine brain membrane extract using GTX30678 LRRK2 antibody.
Loading : 50μg
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GTX30678 IHC-P Image
IHC-P analysis of human brain tissue using GTX30678 LRRK2 antibody.
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GTX30678 IP Image
IP analysis of SH-SY5Y cell lysate using GTX30678 LRRK2 antibody.
Lane 1 : Rabbit IgG
Lane 2 and 3 : GTX30678
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GTX30678 ICC/IF Image
ICC/IF analysis of SH-SY5Y cells using GTX30678 LRRK2 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200
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REFERENCE
There are currently no references for LRRK2 antibody (GTX30678). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LRRK2 antibody (GTX30678). Be the first to share your experience with this product.
