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LSD2 / AOF1 antibody

Anti-LSD2 / AOF1 antibody used in Western Blot (WB). GTX32122

Cat. No. GTX32122

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all LSD2 / AOF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

K562 Cell Lysate(GTX27911)

Product Note

At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KDM1B antibody was raised against a 17 amino acid peptide near the amino terminus of human KDM1B.The immunogen is located within amino acids 80 - 130 of KDM1B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887637

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 1B , AOF1 , C6orf193 , LSD2

Cellular Localization

Nucleus

Background

Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-LSD2 / AOF1 antibody used in Western Blot (WB). GTX32122

GTX32122 WB Image

WB analysis of K562 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32122 KDM1B / AOF1 antibody.
Working concentration : 0.5 μg/ml

REFERENCE

Application Reference

Juan TK et al. Oncol Lett 2018; 15 (5) : 7716-7724 Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-庥B, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways.
Juan TK et al. Oncology Letters 2018; 15 (5) : 7716-7724 Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399