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LSM1 antibody

Anti-LSM1 antibody used in Immunoprecipitation (IP). GTX130240
Anti-LSM1 antibody used in Western Blot (WB). GTX130240
Anti-LSM1 antibody used in Western Blot (WB). GTX130240

Cat No. GTX130240

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , 293T , A431

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human LSM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LSM1 homolog, mRNA degradation associated , CASM , YJL124C

Cellular Localization

Nucleus

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LSM1 antibody used in Immunoprecipitation (IP). GTX130240

GTX130240 IP Image

Immunoprecipitation of LSM1 protein from 293T whole cell extracts using 5 μg of LSM1 antibody (GTX130240).
Western blot analysis was performed using LSM1 antibody (GTX130240).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-LSM1 antibody used in Western Blot (WB). GTX130240

GTX130240 WB Image

LSM1 antibody detects LSM1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LSM1 antibody (GTX130240) diluted at 1:1000.

Anti-LSM1 antibody used in Western Blot (WB). GTX130240

GTX130240 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LSM1 antibody (GTX130240) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169