APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.05-0.2μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88185-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RYKFVATGHGKYEKV, from the C Terminus of the protein sequence according to NP_002330.1; NP_001013271.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lymphocyte specific protein 1 , WP34 , pp52
Background
This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88185 WB Image
WB analysis of Daudi cell ysate (35μg protein in RIPA buffer) using GTX88185 LSP1 antibody, C-term.
Dilution : 0.03μg/ml
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GTX88185 IHC-P Image
IHC-P analysis of paraffin embedded human colon using GTX88185 LSP1 antibody, C-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX88185 WB Image
WB analysis of human PBM (A), Lymph Node (B) and tonsil (C) lysate (35μg protein in RIPA buffer) using GTX88185 LSP1 antibody, C-term.
Dilution : 0.05μg/ml (human PBM) and 0.03μg/ml (human lymph node and tonsil)
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GTX88185 WB Image
WB analysis of human liver lysate (lane 1), mouse liver lysate (lane 2) and rat liver lysate (lane 3) using GTX88185 LSP1 antibody, C-term.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88185 WB Image
WB analysis of mouse spleen lysate (35μg protein in RIPA buffer) using GTX88185 LSP1 antibody, C-term.
Dilution : 0.01μg/ml
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REFERENCE
There are currently no references for LSP1 antibody, C-term (GTX88185). Be the first to share your publications with this product.
REVIEW
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