H1299 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2] (GTX101725) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using LTBP4(GTX101725) antibody at 1:200 dilution.

LTBP4 antibody [N1N2], N-term detects LTBP4 protein at cytosol on human adrenal gland tumor by immunohistochemical analysis.
Sample: Paraffin-embedded adrenal gland tumor.
LTBP4 antibody [N1N2], N-term (GTX101725) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min