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LTBP4 antibody [N1N2], N-term

Orthogonal
Anti-LTBP4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101725
Anti-LTBP4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101725
Anti-LTBP4 antibody [N1N2], N-term used in Western Blot (WB). GTX101725

Cat. No. GTX101725

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all LTBP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Observed MW

210-260 kDa.

Positive Control

Mouse brain , mouse brain

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LTBP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241032

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

latent transforming growth factor beta binding protein 4 , ARCL1C , LTBP-4 , LTBP4L , LTBP4S

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Transforming growth factor-beta, or TGFB (see TGFB1; MIM 190180), remains biologically latent after secretion, blocked from activity by its N-terminal propeptide (the latency-associated peptide, or LAP). TGFB is activated by its dissociation from LAP. The latent form of TGFB is secreted in association with a latent TGFB-binding protein, or LTBP (see LTBP1; MIM 150390), and assembled into the extracellular matrix. LTBPs are mainly composed of epidermal growth factor (EGF; MIM 131530)-like repeats and protein domains with 8-cysteine repeats, both of which are also found in fibrillins (e.g., MIM 134797). See Oklu and Hesketh (2000) [PubMed 11104663] for a review of the LTBP gene family.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LTBP4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101725

GTX101725 IHC-P Image

LTBP4 antibody [N1N2], N-term detects LTBP4 protein at cytosol on human adrenal gland tumor by immunohistochemical analysis.
Sample: Paraffin-embedded adrenal gland tumor.
LTBP4 antibody [N1N2], N-term (GTX101725) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LTBP4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101725

GTX101725 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LTBP4(GTX101725) antibody at 1:200 dilution.

Anti-LTBP4 antibody [N1N2], N-term used in Western Blot (WB). GTX101725

GTX101725 WB Image

Orthogonal

H1299 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2] (GTX101725) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tomasovic A et al. Matrix Biol 2016; Ltbp4 regulates Pdgfrβ expression via TGFβ-dependent modulation of Nrf2 transcription factor function.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169