GeneTex
United States (US)

LTBP4 antibody [N1N2], N-term

GTX101725_43033_20171117_WB_M_brain_19011506_611.jpg
GTX101725_39549_IFA_19011506_394.jpg
GTX101725_43033_20171117_WB_19011506_294.jpg
GTX101725_42214_20150924_WB_M_brain_19011506_914.jpg
GTX101725_39549_IHC_19011506_114.jpg

Cat No. GTX101725

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

173 kDa. ( Note )

Positive Control

K562 , Mouse brain

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LTBP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Latent Transforming Growth Factor Beta Binding Protein 4,Arcl1C,Ltbp-4,Ltbp4L,Ltbp4S,Ltbp4

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Transforming growth factor-beta, or TGFB (see TGFB1; MIM 190180), remains biologically latent after secretion, blocked from activity by its N-terminal propeptide (the latency-associated peptide, or LAP). TGFB is activated by its dissociation from LAP. The latent form of TGFB is secreted in association with a latent TGFB-binding protein, or LTBP (see LTBP1; MIM 150390), and assembled into the extracellular matrix. LTBPs are mainly composed of epidermal growth factor (EGF; MIM 131530)-like repeats and protein domains with 8-cysteine repeats, both of which are also found in fibrillins (e.g., MIM 134797). See Oklu and Hesketh (2000) [PubMed 11104663] for a review of the LTBP gene family.[supplied by OMIM]

Database

DATA IMAGES
GTX101725_43033_20171117_WB_M_brain_19011506_611.jpg

GTX101725 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2], N-term (GTX101725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 27148972

GTX101725_39549_IFA_19011506_394.jpg

GTX101725 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LTBP4(GTX101725) antibody at 1:200 dilution.

GTX101725_43033_20171117_WB_19011506_294.jpg

GTX101725 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2], N-term (GTX101725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 27148972

GTX101725_42214_20150924_WB_M_brain_19011506_914.jpg

GTX101725 WB Image

LTBP4 antibody detects LTBP4 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody (GTX101725) diluted at 1:500.

GTX101725_39549_IHC_19011506_114.jpg

GTX101725 IHC-P Image

LTBP4 antibody [N1N2], N-term detects LTBP4 protein at cytosol on human adrenal gland tumor by immunohistochemical analysis.
Sample: Paraffin-embedded adrenal gland tumor.
LTBP4 antibody [N1N2], N-term (GTX101725) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169