GeneTex
United States (US)

LTBR antibody

Cat No. GTX10493

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
FACS 2.5 μg

Note :

FACS
using 1ng/lane rhTNF RII/TNFRSF1B Fc Chimera under non-reducing conditions

Not tested in other applications.

Calculated MW

47 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

recombinant human lymphotoxin β receptor extracellular domain expressed in NS0 cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphotoxin Beta Receptor,D12S370,Lt-Beta-R,Tnf-R-Iii,Tnfcr,Tnfr-Rp,Tnfr2-Rp,Tnfr3,Tnfrsf3,Ltbr

Background

Tumor necrosis factor-beta (TNF-beta), also known as lymphotoxin (LT or LT-a) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-beta and TNF-alpha share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-alpha, TNF-beta can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-beta (LT-beta). Reportedly, LT-alpha1/beta2, the predominant heterotrimer, binds and activates only the LT-beta receptor, LT-betaR. The other heterotrimer, LT-alpha2/beta1 binds and activates TNFR1 and TNFR2 but not LT-betaR. TNF-beta is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-beta, LT-beta and TNF-alpha are tightly linked near each other within the MHC. TNF-beta appears to play many of the same biological roles as TNF-alpha, but the membrane-bound form with LT-beta is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-alpha and TNF-beta have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-beta are 74% homologous and human and mouse LT-beta are 80% homologous.

Database

Research Area

Package List Price ($)
$ 429