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LXN antibody [N1C3]

KO/KD
Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116
Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116
Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116
Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116
Anti-LXN antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107116
Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116
Anti-LXN antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107116

Cat. No. GTX107116

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all LXN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Mouse brain , 293T , HeLaS3 , rat brain , rat heart , HeLa

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LXN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950842

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

latexin , ECI , TCI

Cellular Localization

Cytoplasm

Background

This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116

GTX107116 WB Image

KO/KD

Wild-type (WT) and LXN knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody [N1C3] (GTX107116) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116

GTX107116 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody [N1C3] (GTX107116) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116

GTX107116 WB Image

Sample(30 μg whole cell lysate)
A:293T
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX107116 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116

GTX107116 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody [N1C3] (GTX107116) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LXN antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107116

GTX107116 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using LXN(GTX107116) antibody at 1:200 dilution.

Anti-LXN antibody [N1C3] used in Western Blot (WB). GTX107116

GTX107116 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX107116 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LXN antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107116

GTX107116 IHC-P Image

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using LXN(GTX107116) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169