APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89661-PEP).
Product Note
Based on the peptide used, this antibody is expected to be specific for LXR beta and not to cross-react with LXR alpha.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence SSPTTSSLDTPLPGC, from the N Terminus of the protein sequence according to NP_009052.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
RRID
AB_10725130
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor subfamily 1 group H member 2 , LXR-b , LXRB , NER , NER-I , RIP15 , UNR
Cellular Localization
Nucleus
Background
The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]
Database
Research Area
DATA IMAGES
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GTX89661 IHC-P Image
IHC-P analysis of human spleen using GTX89661 LXR beta antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3μg/ml
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GTX89661 WB Image
WB analysis of human bone marrow lysate using GTX89661 LXR beta antibody, N-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89661 IHC-P Image
IHC-P analysis of human uterus using GTX89661 LXR beta antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3μg/ml
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GTX89661 WB Image
The data was published in the journal PLoS One in 2014.PMID: 25184494
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REFERENCE
REVIEW
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