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LXR beta antibody [N2C2], Internal

Anti-LXR beta antibody [N2C2], Internal used in Western Blot (WB). GTX113571
Anti-LXR beta antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113571
Anti-LXR beta antibody [N2C2], Internal used in Western Blot (WB). GTX113571

Cat No. GTX113571

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa , mouse brain

Predict Reactivity

Rat, Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LXR beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037548

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor subfamily 1 group H member 2 , LXR-b , LXRB , NER , NER-I , RIP15 , UNR

Cellular Localization

Nucleus

Background

The LX receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor (see MIM 180245) and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides. Two genes, alpha (LXRA, MIM 602423) and beta, are known to encode LXR proteins (Song et al., 1995 [PubMed 7625741]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LXR beta antibody [N2C2], Internal used in Western Blot (WB). GTX113571

GTX113571 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX113571 diluted at 1:1000

Anti-LXR beta antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113571

GTX113571 ICC/IF Image

LXR beta antibody [N2C2], Internal detects LXR beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LXR beta protein stained by LXR beta antibody [N2C2], Internal (GTX113571) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-LXR beta antibody [N2C2], Internal used in Western Blot (WB). GTX113571

GTX113571 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113571 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169