Non-transfected (–) and transfected (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

LY6E antibody [HL1933] detects LY6E protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min