GeneTex
  • Country / Location Selection

United States (US)

LYAG antibody [C2C3], C-term

Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821
Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821
Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821
Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821

Cat No. GTX109821

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LYAG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950323

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glucosidase alpha, acid , LYAG

Cellular Localization

Lysosome

Background

This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821

GTX109821 WB Image

Wild-type (WT) and LYAG knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821

GTX109821 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821

GTX109821 IHC-P Image

LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821

GTX109821 IHC-P Image

LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

There are currently no reviews for LYAG antibody [C2C3], C-term (GTX109821). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf