GeneTex
United States (US)

LYK5 antibody

GTX107557_40737_WB_M_Brain_19011506_220.jpg
GTX107557_40737_IFA_19011506_316.jpg
GTX107557_40737_IHC_19011506_358.jpg
GTX107557_40737_WB_19011506_944.jpg

Cat No. GTX107557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , HepG2 , mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYK5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ste20-Related Kinase Adaptor Alpha,Lyk5,Ny-Br-96,Pmse,Strad,Strad Alpha,Stlk,Strada

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107557_40737_WB_M_Brain_19011506_220.jpg

GTX107557 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX107557 diluted at 1:1000

GTX107557_40737_IFA_19011506_316.jpg

GTX107557 ICC/IF Image

LYK5 antibody detects LYK5 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LYK5 protein stained by LYK5 antibody (GTX107557) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107557_40737_IHC_19011506_358.jpg

GTX107557 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using LYK5(GTX107557) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107557_40737_WB_19011506_944.jpg

GTX107557 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
12% SDS PAGE
GTX107557 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169