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LYN antibody [N2C3]

Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543
Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543
Anti-LYN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111543
Anti-LYN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111543
Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543

Cat No. GTX111543

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

A431 , mouse brain , K562

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950843

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

Database

Research Area

DATA IMAGES

Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543

GTX111543 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX111543 diluted at 1:1000

Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543

GTX111543 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111543 diluted at 1:1000

Anti-LYN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111543

GTX111543 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LYN(GTX111543) antibody at 1:500 dilution.

Anti-LYN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111543

GTX111543 IHC-P Image

LYN antibody [N2C3] detects LYN protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYN antibody [N2C3] (GTX111543) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
The observed location is based on the publication: PMID: 18817770

Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543

GTX111543 WB Image

LYN antibody detects LYN protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX111543) at a dilution of 1:1000.

REFERENCE

There are currently no references for LYN antibody [N2C3] (GTX111543). Be the first to share your publications with this product.

REVIEW

LYN antibody [N2C3] Cat No. GTX111543

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169