LYN antibody [N2C3]

Name: LYN antibody [N2C3]
Brand: GeneTex
SKU: GTX111543
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543

Anti-LYN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111543

Anti-LYN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111543

Cat. No. GTX111543

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all LYN products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Mouse brain , A431 , K562

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950843

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

Database

Gene ID: 4067 LYN
UniProt: P07948 LYN

Research Area

DATA IMAGES

GTX111543 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX111543 diluted at 1:1000

GTX111543 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111543 diluted at 1:1000

GTX111543 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LYN(GTX111543) antibody at 1:500 dilution.

GTX111543 IHC-P Image

LYN antibody [N2C3] detects LYN protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYN antibody [N2C3] (GTX111543) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
The observed location is based on the publication: PMID: 18817770

GTX111543 WB Image

LYN antibody detects LYN protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX111543) at a dilution of 1:1000.

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REVIEW