LYN antibody [N2C3]

Name: LYN antibody [N2C3]
Brand: GeneTex
SKU: GTX111543
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-LYN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111543

Anti-LYN antibody [N2C3] used in Western Blot (WB). GTX111543

Anti-LYN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX111543

Cat. No. GTX111543

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all LYN products

Datasheet
Document

View product citations for antibody GTX111543 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Mouse brain , A431 , K562

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950843

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

Database

Gene ID: 4067 LYN
UniProt: P07948 LYN

Research Area

DATA IMAGES

GTX111543 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LYN(GTX111543) antibody at 1:500 dilution.

GTX111543 WB Image

LYN antibody detects LYN protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX111543) at a dilution of 1:1000.

GTX111543 IHC-P Image

LYN antibody [N2C3] detects LYN protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYN antibody [N2C3] (GTX111543) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
The observed location is based on the publication: PMID: 18817770

GTX111543 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX111543 diluted at 1:1000

GTX111543 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111543 diluted at 1:1000

REFERENCE

There are currently no references for LYN antibody [N2C3] (GTX111543). Be the first to share your publications with this product.

Submit a Reference

REVIEW