GeneTex
United States (US)

LYN antibody

Orthogonal
GTX101222_40373_20180824_WB_KO_watermark_19011506_216.jpg
GTX101222_40373_WB_19011506_170.jpg
GTX101222_40373_IP_19011506_133.jpg
GTX101222_40373_IFA_19011506_617.jpg
GTX101222_40373_IHC_19011506_840.jpg

Cat No. GTX101222

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HeLa , CD44-knockout HeLa , Raji , K562 , THP-1

Predict Reactivity

Rat, Sheep, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lyn Proto-Oncogene, Src Family Tyrosine Kinase,Jtk8,P53Lyn,P56Lyn,Lyn

Database

Research Area

DATA IMAGES
GTX101222_40373_20180824_WB_KO_watermark_19011506_216.jpg

GTX101222 WB Image

Orthogonal

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX101222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed effect is based on the publication: PMID: 17599831

GTX101222_40373_WB_19011506_170.jpg

GTX101222 WB Image

LYN antibody detects LYN protein by Western blot analysis.
A. 30 µg Raji whole cell lysate/extract
B. 30 µg K562 whole cell lysate/extract
C. 30 µg Raji whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (GTX101222) dilution: 1:1000

GTX101222_40373_IP_19011506_133.jpg

GTX101222 IP Image

LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of LYN protein by 4 μg LYN antibody (GTX101222)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (GTX101222) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX101222_40373_IFA_19011506_617.jpg

GTX101222 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX101222) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101222_40373_IHC_19011506_840.jpg

GTX101222 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LYN(GTX101222) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169