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LYN antibody

KO/KD Orthogonal
Anti-LYN antibody used in Western Blot (WB). GTX111584
Anti-LYN antibody used in Immunoprecipitation (IP). GTX111584
Anti-LYN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111584
Anti-LYN antibody used in Western Blot (WB). GTX111584

Cat No. GTX111584

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($379),
25 μl ($169)
See all LYN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HeLa , THP-1 , CD44-knockout HeLa

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164763

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

Database

Research Area

DATA IMAGES

Anti-LYN antibody used in Western Blot (WB). GTX111584

GTX111584 WB Image

KO/KD Orthogonal

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX111584) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed effect is based on the publication: PMID: 17599831

Anti-LYN antibody used in Immunoprecipitation (IP). GTX111584

GTX111584 IP Image

LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. 40 μg K562 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of LYN protein by 4 μg LYN antibody (GTX111584)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (GTX111584) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-LYN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111584

GTX111584 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX111584) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-LYN antibody used in Western Blot (WB). GTX111584

GTX111584 WB Image

LYN antibody detects LYN protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (GTX111584) dilution: 1:1000

REFERENCE

Application Reference

Morinaga T et al. Sci Rep 2017; 7 (1) : 493 Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells.
Morinaga T et al. J Biol Chem 2014; (Epub) Activation of Lyn Tyrosine Kinase through Decreased Membrane Cholesterol Levels During a Change in its Membrane Distribution upon Cell Detachment.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169