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LYN antibody

KO/KD Orthogonal
Anti-LYN antibody used in Western Blot (WB). GTX111584
Anti-LYN antibody used in Immunoprecipitation (IP). GTX111584
Anti-LYN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111584
Anti-LYN antibody used in Western Blot (WB). GTX111584

Cat. No. GTX111584

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LYN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

THP-1 , HeLa

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

Database

Research Area

DATA IMAGES

Anti-LYN antibody used in Western Blot (WB). GTX111584

GTX111584 WB Image

KO/KD Orthogonal

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX111584) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed effect is based on the publication: PMID: 17599831

Anti-LYN antibody used in Immunoprecipitation (IP). GTX111584

GTX111584 IP Image

LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. 40 μg K562 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of LYN protein by 4 μg LYN antibody (GTX111584)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (GTX111584) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-LYN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111584

GTX111584 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX111584) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-LYN antibody used in Western Blot (WB). GTX111584

GTX111584 WB Image

LYN antibody detects LYN protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (GTX111584) dilution: 1:1000

REFERENCE

Application Reference

Morinaga T et al. Sci Rep 2017; 7 (1) : 493 Recruitment of Lyn from endomembranes to the plasma membrane through calcium-dependent cell-cell interactions upon polarization of inducible Lyn-expressing MDCK cells.
Morinaga T et al. J Biol Chem 2014; (Epub) Activation of Lyn Tyrosine Kinase through Decreased Membrane Cholesterol Levels During a Change in its Membrane Distribution upon Cell Detachment.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169