APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr397 of human LYN. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888566
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn
Cellular Localization
Cell membrane,Nucleus,Cytoplasm,Golgi apparatus,Membrane
Background
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Database
Research Area
DATA IMAGES
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/templates/default/js/lightbox/images/loading.gif "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
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GTX635694 WB Image
Untreated (–) and treated (+) Raji whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody (GTX635694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635694 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635694 IHC-P Image
LYN (phospho Tyr397) antibody [HL122] detects LYN (phospho Tyr397) protein in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.Sample: Paraffin-embedded Human Matched Pair Tissue Array - Brain (Alzheimer's Disease + Normal) (GTX49382).LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635694 IHC-P Image
LYN (phospho Tyr397) antibody detects LYN (phospho Tyr397) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody (GTX635694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The expression pattern is based on the publication:PMID: 25409824
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REFERENCE
REVIEW
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![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44027_20201008_WB_treatment_Pervanadate_20101601_988.jpg "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44027_20220128_WB_treatment_Pervanadate_peptideblocking_22020818_665.jpg "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44027_20220218_IHC-P_22030201_527.jpg "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44683_20221227_IHC-P_multiple_M_22122821_597.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44027_20201008_WB_treatment_Pervanadate_20101601_988.jpg "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44027_20220128_WB_treatment_Pervanadate_peptideblocking_22020818_665.jpg "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44027_20220218_IHC-P_22030201_527.jpg "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44683_20221227_IHC-P_multiple_M_22122821_597.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")