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LYN (phospho Tyr397) antibody [HL122]

Recombinant antibody Orthogonal
Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694
Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694
Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694

Cat No. GTX635694

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL122

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($379),
25 μl ($169)
See all LYN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr397 of human LYN. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888566

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

Cellular Localization

Cell membrane,Nucleus,Cytoplasm,Golgi apparatus,Membrane

Background

This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694

GTX635694 WB Image

Orthogonal

Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694

GTX635694 IHC-P Image

Orthogonal

LYN (phospho Tyr397) antibody [HL122] detects LYN (phospho Tyr397) protein in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.
Sample: Paraffin-embedded Human Matched Pair Tissue Array - Brain (Alzheimer's Disease + Normal) (GTX49382).
LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694

GTX635694 WB Image

Orthogonal

Untreated (–) and treated (+) Raji whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody (GTX635694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Munshi M et al. Blood Adv 2022; A new role for the SRC family kinase HCK as a driver of SYK activation in MYD88 mutated lymphomas.
Application : WB
Reactivity : Human
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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations