APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr397 of human LYN. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888566
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn
Cellular Localization
Cell membrane,Nucleus,Cytoplasm,Golgi apparatus,Membrane
Background
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Database
Research Area
DATA IMAGES
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/templates/default/js/lightbox/images/loading.gif "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
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GTX635694 IHC-P Image
LYN (phospho Tyr397) antibody [HL122] detects LYN (phospho Tyr397) protein in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.Sample: Paraffin-embedded Human Matched Pair Tissue Array - Brain (Alzheimer's Disease + Normal) (GTX49382).LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635694 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635694 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635694 IHC-P Image
LYN (phospho Tyr397) antibody detects LYN (phospho Tyr397) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody (GTX635694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The expression pattern is based on the publication:PMID: 25409824
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REFERENCE
REVIEW
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![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44027_20220218_IHC-P_w_23061202_348.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44027_20220128_WB_treatment_Pervanadate_peptideblocking_w_23061202_906.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44683_20220520_WB_treatment_pervanadate_23013122_283.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/small/GTX635694/GTX635694_44683_20221227_IHC-P_multiple_M_22122821_597.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44027_20220218_IHC-P_w_23061202_348.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44027_20220128_WB_treatment_Pervanadate_peptideblocking_w_23061202_906.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44683_20220520_WB_treatment_pervanadate_23013122_283.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in Western Blot (WB). GTX635694")
![Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694](/upload/website/prouct_img/normal/GTX635694/GTX635694_44683_20221227_IHC-P_multiple_M_22122821_597.webp "Anti-LYN (phospho Tyr397) antibody [HL122] used in IHC (Paraffin sections) (IHC-P). GTX635694")