GeneTex
United States (US)

LYRIC antibody [N2C3]

GTX100587_41045_IHC_19011506_252.jpg
GTX100587_41045_IHC_2_19011506_543.jpg
GTX100587_39911_WB_R_brain_19011506_660.jpg
GTX100587_41045_IHC_M_19011506_867.jpg
GTX100587_41045_IFA_19011506_517.jpg

Cat No. GTX100587

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

rat brain

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYRIC . The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

3D3 antibody, AEG-1 antibody, AEG1 antibody, LYRIC antibody, LYRIC/3D3 antibody, MTDH antibody, protein LYRIC antibody, astrocyte elevated gene-1 protein antibody, metastasis adhesion protein antibody, 3D3/LYRIC antibody, lysine-rich CEACAM1 co-isolated protein antibody, metadherin antibody

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein (By similarity) , Cell junction , tight junction (By similarity) , Nucleus , nucleolus (By similarity) , Cytoplasm , perinuclear region

Background

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Database

Research Area

DATA IMAGES
GTX100587_41045_IHC_19011506_252.jpg

GTX100587 IHC-P Image

LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYRIC antibody [N2C3] (GTX100587) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100587_41045_IHC_2_19011506_543.jpg

GTX100587 IHC-P Image

LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
LYRIC antibody [N2C3] (GTX100587) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100587_39911_WB_R_brain_19011506_660.jpg

GTX100587 WB Image

LYRIC antibody detects LYRIC protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LYRIC antibody (GTX100587) diluted by 1:1000.
Observed TARGET protein has mutiple bands at 68 kDa, and 80 kDa, possibly due to alternative splicing. Reference: Biochem Biophys Res Commun. 2008 Jun 27;371(2):333-8.

GTX100587_41045_IHC_M_19011506_867.jpg

GTX100587 IHC-P Image

LYRIC antibody [N2C3] detects LYRIC protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
LYRIC antibody [N2C3] (GTX100587) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100587_41045_IFA_19011506_517.jpg

GTX100587 ICC/IF Image

LYRIC antibody [N2C3] detects LYRIC protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: LYRIC protein stained by LYRIC antibody [N2C3] (GTX100587) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf