GeneTex
United States (US)

Lactate Dehydrogenase antibody (HRP)

Lactate-Dehydrogenase-antibody-HRP-GTX27639-ICC_IF-1_18121411_183.jpg
Lactate-Dehydrogenase-antibody-HRP-GTX27639-WB-1_18121411_647.jpg

Cat No. GTX27639

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA, IHC
Reactivity Human, Mouse, Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin and 0.01% gentamicin sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Full length native Lactate Dehydrogenase (purified)

Purification

IgG fraction

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LDHM

Cellular Localization

Cytoplasmic

Background

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH-1 to LDH-5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream).

Research Area

DATA IMAGES
Lactate-Dehydrogenase-antibody-HRP-GTX27639-ICC_IF-1_18121411_183.jpg

GTX27639 ICC/IF Image

GeneTex biotin-conjugated anti-lactate dehydrogenase antibody was used to stain MeOH/Acetone-fixed HeLa cells by immunofluorescence.

Lactate-Dehydrogenase-antibody-HRP-GTX27639-WB-1_18121411_647.jpg

GTX27639 WB Image

Western blot analysis shows GeneTex's anti-lactate dehydrogenase antibody detects LDH in HeLa cell extracts.  Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 µg was loaded per lane onto a 10% gel for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1,000  dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.