GeneTex

  • Country / Location Selection

United States (US)

Lactate Dehydrogenase antibody

Orthogonal
Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101
Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101
Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101
Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101

Cat. No. GTX22101

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Rabbit
Package
1 mg ($399)
See all Lactate Dehydrogenase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:200
IP 1:100
ELISA 1:2000-1:10000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Full length lactate dehydrogenase protein isolated from rabbit muscle.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactate dehydrogenase A , LDH-M

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step
of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase
family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding
different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of
this gene. (provided by RefSeq)

Database

Research Area

DATA IMAGES

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101

GTX22101 WB Image

Western blot analysis of anti-Lactate Dehydrogenase antibody (1:1000 dilution) in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). A single band of ~36 kDa corresponds to the expected molecular weight for the protein. Personal communication Kuldeep Patel.

Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101

GTX22101 ICC/IF Image

GeneTex biotinylated anti-lactate dehydrogenase antibody was used to stain MeOH/Acetone-fixed HeLa cells by immunofluorescence.

Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22101

GTX22101 ICC/IF Image

ICC/IF analysis of MeOH-fixed HeLa cells using GTX22101 Lactate Dehydrogenase antibody.
Dilution : 1:200

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX22101

GTX22101 WB Image

Orthogonal

WB analysis of HeLa whole cell lysate using GTX22101 Lactate Dehydrogenase antibody.
Lane 1-4 : Cytoplasmic extracts (CF)
Lane 5-8 : Nuclear extracts (NF)
Loading : 30 μg
Dilution : 1:400

REFERENCE

Clone Reference

Crawford RM et al. EMBO J 2002; 21 (15):3936-48 M-LDH serves as a sarcolemmal K(ATP) channel subunit essential for cell protection against ischemia.
Submit a Reference

REVIEW

There are currently no reviews for Lactate Dehydrogenase antibody (GTX22101). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399