Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rabbit
Immunogen
Lactate Dehydrogenase [Rabbit Muscle]
Purification
IgG fraction
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lactate dehydrogenase A , LDH-M
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. (provided by RefSeq)
GeneTex biotinylated anti-lactate dehydrogenase antibody was used to stain MeOH/Acetone-fixed HeLa cells by immunofluorescence.
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GTX22101 WB Image
Western blot analysis of anti-Lactate Dehydrogenase antibody (1:1000 dilution) in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). A single band of ~36 kDa corresponds to the expected molecular weight for the protein. Personal communication Kuldeep Patel.
