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Lactate Dehydrogenase antibody

Orthogonal
Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX48868
Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48868
Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX48868

Cat. No. GTX48868

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Lactate Dehydrogenase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF Assay dependent
IP 1:100
ELISA 1:5000-1:20000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Full length lactate dehydrogenase protein isolated from rabbit muscle.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Research Area

DATA IMAGES

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX48868

GTX48868 WB Image

Western blot analysis shows GeneTex's Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). 30 μg was loaded per lane. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with 1:400 for overnight at 4ºC. Secondary antibody at 1:10,000 for 45 min at RT.

Anti-Lactate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48868

GTX48868 ICC/IF Image

ICC/IF analysis of MeOH-fixed HeLa cells using GTX48868 Lactate Dehydrogenase antibody.
Dilution : 1:200

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX48868

GTX48868 WB Image

Orthogonal

WB analysis of HeLa whole cell lysate using GTX48868 Lactate Dehydrogenase antibody.
Lane 1-4 : Cytoplasmic extracts
Lane 5-8 : Nuclear extracts
Loading : 30 μg
Dilution : 1:400

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399