GeneTex
  • Country / Location Selection

United States (US)

Lactate Dehydrogenase antibody

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX48868

Cat No. GTX48868

Host

Goat

Clonality

Polyclonal

Application

WB, IP, ELISA

Reactivity

Human, Rabbit
Package
100 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:100
ELISA 1:4000 - 1:20000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Research Area

DATA IMAGES

Anti-Lactate Dehydrogenase antibody used in Western Blot (WB). GTX48868

GTX48868 WB Image

Western blot analysis shows GeneTex's Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). 30 μg was loaded per lane. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with 1:400 for overnight at 4ºC. Secondary antibody at 1:10,000 for 45 min at RT.

REFERENCE

There are currently no references for Lactate Dehydrogenase antibody (GTX48868). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Lactate Dehydrogenase antibody (GTX48868). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf