APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.05-0.2μg/ml |
| 2-4μg/ml |
| Assay dependent |
|
Note :
IHC-P
Blood vessel shows strong staining of cytoplasm of neutrophils..
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88565-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-ENYKSQQSSDPDP, from the internal region of the protein sequence according to NP_002334.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lactotransferrin , GIG12 , HEL110 , HLF2 , LF
Cellular Localization
Nucleus
Background
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Database
Research Area
DATA IMAGES
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GTX88565 IHC-P Image
IHC-P analysis of human blood vessel using GTX88565 Lactoferrin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
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GTX88565 WB Image
WB analysis of peripheral blood mononucleocyte lysate using GTX88565 Lactoferrin antibody, Internal.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88565 ELISA Image
ELISA analysis of Lactoferrin protein using GTX88565 Lactoferrin antibody, Internal. This antibody was used as a detecting antibody.
Dilution : 5μg/ml
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REFERENCE
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REVIEW
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