GeneTex
United States (US)

Lactoferrin antibody, Internal

GTX88565_920_IHC-P_18121411_176.jpg
GTX88565_2249_WB_18121411_974.jpg
GTX88565_17_ELISA_18121411_496.jpg

Cat No. GTX88565

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.05-0.2μg/ml
IHC-P 2-4μg/ml
ELISA Assay dependent

Note :

IHC-P
Blood vessel shows strong staining of cytoplasm of neutrophils..

Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88565-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ENYKSQQSSDPDP, from the internal region of the protein sequence according to NP_002334.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lactotransferrin,Gig12,Hel110,Hlf2,Lf,Ltf

Cellular Localization

Secreted

Background

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES
GTX88565_920_IHC-P_18121411_176.jpg

GTX88565 IHC-P Image

IHC-P analysis of human blood vessel using GTX88565 Lactoferrin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2µg/ml

GTX88565_2249_WB_18121411_974.jpg

GTX88565 WB Image

WB analysis of peripheral blood mononucleocyte lysate using GTX88565 Lactoferrin antibody, Internal.
Dilution : 0.05µg/ml
Loading : 35µg protein in RIPA buffer

GTX88565_17_ELISA_18121411_496.jpg

GTX88565 ELISA Image

ELISA analysis of Lactoferrin protein using GTX88565 Lactoferrin antibody, Internal. This antibody was used as a detecting antibody.
Dilution : 5µg/ml

Package List Price ($)
$ 329