GeneTex
United States (US)

Lactoferrin antibody

Lactoferrin-antibody_IHC_GTX15811-1_18121411_647.jpg

Cat No. GTX15811

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, IHC
Reactivity Human
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at a dilution of >1/200. WB: Use at a dilution of >1/500. Predicted molecular weight: 79 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

78 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Antibody stabilization buffer.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75-1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length native protein (purified) (Human).

Purification

Immunogen affinity purified
Affinity purified using immobilized antigenic proteins and pre adsorbed on an immobilized heterologus lactoferin matrix to remove the cross reacting antibodies.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lactotransferrin,Gig12,Hel110,Hlf2,Lf,Ltf

Cellular Localization

Secreted

Background

Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

Database

Research Area

DATA IMAGES
Lactoferrin-antibody_IHC_GTX15811-1_18121411_647.jpg

GTX15811 IHC Image

Thin section of human intestine with inflammatory infilterate. Antibody GTX15811 (1:500) diluted in diluObuffer.

Package List Price ($)
$ 359