GeneTex
United States (US)

Laforin antibody [C2C3], C-term

GTX104648_39638_IFA_19011506_349.jpg
GTX104648_39638_WB_19011506_444.jpg
GTX104648_WB_19011506_511.jpg
GTX104648_39638_IHC_19011506_582.jpg

Cat No. GTX104648

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Laforin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EPM2 antibody, MELF antibody, EPM2A antibody, lafora PTPase antibody, laforin antibody, "epilepsy, progressive myoclonus type 2, Lafora disease (laforin) antibody", LAFPTPase antibody, "epilepsy, progressive myoclonus type 2A, Lafora disease (laforin) antibody"

Cellular Localization

Cytoplasm , Isoform 1: Endoplasmic reticulum , Cell membrane , Isoform 2: Endoplasmic reticulum , Nucleus

Background

This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104648_39638_IFA_19011506_349.jpg

GTX104648 ICC/IF Image

Laforin antibody [C2C3], C-term detects EPM2A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EPM2A protein stained by Laforin antibody [C2C3], C-term (GTX104648) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX104648_39638_WB_19011506_444.jpg

GTX104648 WB Image

Laforin antibody detects Laforin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Laforin antibody (GTX104648) diluted by 1:3000.

GTX104648_WB_19011506_511.jpg

GTX104648 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
12% SDS PAGE
GTX104648 diluted at 1:5000

GTX104648_39638_IHC_19011506_582.jpg

GTX104648 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Laforin(GTX104648) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf