GeneTex
United States (US)

Laforin antibody, Internal

GTX88479_867_IHC-P_18122102_560.jpg
GTX88479_2161_WB_18122102_713.jpg

Cat No. GTX88479

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 2.5-3.8μg/ml

Note :

IHC-P
Human Cerebellum shows pixulate staining in the cells of the granular layer.

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88479-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform a (NP_005661.1) only.

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EATGHTNEMKHTTD, from the internal region of the protein sequence according to NP_005661.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epm2A, Laforin Glucan Phosphatase,Epm2,Melf,Epm2A

Cellular Localization

Under glycogenolytic conditions localizes to the nucleus. Primarily associated with polyribosomes at the endoplasmic reticulum, also found at the plasma membrane. Also found in the nucleus.

Background

This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88479_867_IHC-P_18122102_560.jpg

GTX88479 IHC-P Image

IHC-P analysis of human cerebellum using GTX88479 Laforin antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88479_2161_WB_18122102_713.jpg

GTX88479 WB Image

WB analysis of human cerebellum lysate using GTX88479 Laforin antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329