APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1 - 2 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
SW480 Cell Lysate(GTX23957)
Product Note
At least four isoforms of EPM2A are known to exist; this antibody will detect all but the shortest isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Laforin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Laforin.The immunogen is located within amino acids 190 - 240 of Laforin.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
EPM2A glucan phosphatase, laforin , EPM2 , MELF
Cellular Localization
Cytoplasm,Endoplasmic reticulum membrane,Cell membrane,Nucleus
Background
This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31495 WB Image
WB analysis of SW480 cell lysate using GTX31495 Laforin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31495 ICC/IF Image
ICC/IF analysis of SW480 cells using GTX31495 Laforin antibody.
Working concentration : 5 μg/ml
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GTX31495 ICC/IF Image
ICC/IF analysis of SW480 cells using GTX31495 Laforin antibody.
Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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